Investigating the Interaction of Inflammation-Associated Fibroblasts (IAFs) with Colon Epithelial Cells in Inflammatory Bowel Disease (IBD)

نویسندگان

چکیده

Abstract Inflammatory Bowel Disease (IBD) is a chronic inflammatory disease that affects the epithelial lining of gastrointestinal tract. Recent single cell RNA-sequencing (scRNA-seq) studies suggested inflammation-associated fibroblasts (IAFs) play important roles in pathogenesis and drug resistance. To elucidate IAF’s role IBD, we isolated from patients with IBD profiled their responses to panel pro-inflammatory cytokines. We found cocktail cytokines can induce IAF marker genes patient-derived fibroblasts. These cytokine-stimulated recapitulate many characteristics IAFs, including increased ECM chemokine secretion. Furthermore, when co-cultured colon organoids, secrete prostaglandin E2 (PGE2) organoid swelling through EP4-cAMP-PKA-CFTR signaling, which mimics diarrhea crypt distortion IBD. rapid increases chance mitosis error activates p53 pathway, risk tumorigenesis. Thus, are reporting novel mechanisms regulates This study was funded by Takeda Development Center Americas, Inc.

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ژورنال

عنوان ژورنال: Journal of Immunology

سال: 2023

ISSN: ['1550-6606', '0022-1767']

DOI: https://doi.org/10.4049/jimmunol.210.supp.61.03